Using Culture and PCR Technique for Detection of Escherichia coli O157:H7 in Duhok Cheese
نویسندگان
چکیده
منابع مشابه
Detection of Viable But Non-Culturable State of Escherichia coli O157:H7 Using Reverse Transcription PCR
Background and Aims: Many bacteria including Escherichia coli may enter into a viable but non-culturable (VBNC) state under unfavorable stresses, which are unable to be detected by culture-based methods. In this study, the use of Reverse Transcription PCR (RT-PCR) for detection of VBNC state of E. coli O157:H7 was investigated. Materials and Methods: Escherichia. coli O157:H7 was inoculated i...
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Escheric hia coli O157:H7 lives in the intestines of healthy cattle, and can contaminate meat during slaughtering pr actices . Detection of the low infectious dosage of bacterium requires a sensitive method. We developed polymerase chain reaction (PCR) assays to detect the gene Stx2 irrespective of the bacterial serotype. In this study, the detection limit of the PCR protocol in detecting Stx...
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ژورنال
عنوان ژورنال: Kahramanmaraş Sütçü İmam Üniversitesi Doğa Bilimleri Dergisi
سال: 2015
ISSN: 1309-1743
DOI: 10.18016/ksujns.94848